Figure 2.

Normal function of perforin with a valine substitution at residue 211. The line graph shows the lytic activity of A211V perforin observed in ⁵¹Cr-release cytotoxicity assays using transfected RBL cells and Jurkat target cells at the E/T ratios indicated, and compares the putative perforin mutation A211V with WT human perforin and a mutated control perforin. Data shown are means ± SDs. Experiments were repeated three times.