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This article is part of the supplement: Familial Aspects of Cancer 2011 Research and Practice

Open Access Meeting abstract

Functional polymorphisms in the TERT promoter are associated with risk of serious ovarian and breast cancer

Jonathan Beesley18*, Hilda Pickett23, Sharon Johnatty1, Xiaoqing Chen1, Jun Jun Li1, David Rider4, Michael Stutz23, Diether Lambrecht56, Jenny Chang-Claude7, Thilo Dork9, Marc Goodman10, Bart Kiemmney111213, Elisa Bandera14, Linda Kelemen15, Shan Wang-Gorke6, Ian Campbell16, Simon Gayther17, Susan Ramus17, Ellen Goode4, Roger Reddel23, Georgia Chenevix-Trench1, kConFab Investigators and Australian Ovarian Cancer Study Group

  • * Corresponding author: Jonathan Beesley

Author Affiliations

1 Division of Genetics and Population Health, Queensland Institute of Medical Research, Brisbane, Queensland, Australia

2 Cancer Research Unit, Children's Medical Research Institute, Westmead, New South Wales, Australia

3 Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia

4 Department of Health Sciences Research, Mayo Clinic College of Medicine, Rochester, Minnesota, USA

5 Vesalius Research Center, VIB, Leuven, Belgium

6 Vesalius Research Center, University of Leuven, Leuven, Belgium

7 Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany

8 Department of Obstetrics and Gynecology, University of Ulm, Ulm, Germany

9 Clinics of Obstetrics and Gynaecology, Hannover Medical School, Hannover, Germany

10 Cancer Epidemiology Program, University of Hawaii Cancer Center, Honolulu, Hawaii, USA

11 Department of Epidemiology, Biostatistics and HTA, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

12 Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

13 Comprehensive Cancer Center, The Netherlands, Nijmegen, The Netherlands

14 The Cancer Institute of New Jersey/Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA

15 Department of Population Health Research, Alberta Health Services-Cancer Care, Calgary, Alberta, Canada

16 Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia

17 Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, USA

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Hereditary Cancer in Clinical Practice 2012, 10(Suppl 2):A86  doi:10.1186/1897-4287-10-S2-A86

The electronic version of this article is the complete one and can be found online at: http://www.hccpjournal.com/content/10/S2/A86


Published:12 April 2012

© 2012 Beesley et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Meeting abstract

Genome-wide association studies have implicated the TERT-CLPTM1L locus at 5p15.33 in susceptibility to a variety of cancers including pancreas, lung, skin and glioma, suggesting that TERT may act as a “pan-cancer susceptibility locus” in a similar manner to the 8q24 region.

We initially identified an association between an intronic TERT SNP rs7726159 and epithelial ovarian cancer (EOC) risk through an Illumina GoldenGate scan of single nucleotide polymorphisms (SNPs) in candidate genes, with a more pronounced effect in serous cases (Johnatty et al, PloS Genetics 2010). We employed a fine-mapping strategy of a 500 kb region of the TERT-CLPTM1L locus using data from the 1000Genomes and HapMap Projects, and cases and controls from the Ovarian Cancer Association Consortium (OCAC). Using single-marker and stepwise logistic regression adjusted for age and study, we analysed 28 SNPs in 2,130 invasive epithelial ovarian cancer cases, including 1,076 of serous histology, and 3,975 controls of Caucasian ancestry from nine OCAC studies, and observed a significant association between serous cases and a TERT promoter SNP rs2736109 [adj. ORper-allele 0.86 (0.77-0.96), P = 0.005].

Since much of the genetic architecture is shared between EOC and breast cancer, we analysed rs2736109 in 4,277 invasive breast cancer cases and 7,000 controls from the Breast Cancer Association Consortium (BCAC). Although there was no association with invasive breast cancer risk overall [adj. ORper-allele = 0.95 (0.90 - 1.01) P = 0.10], we found the strongest evidence of association among ER-negative cases over the age of 50 (n=636) [adj. ORper-allele 0.84 (0.75-0.95), P = 0.005].

To examine the potential functional consequences of rs2736109 and another promoter SNP, rs2736108, we generated luciferase reporter constructs comprising 3.7 kb of the TERT promoter containing various combinations of alleles and transfected them into breast and ovarian cell lines. We observed a decrease in luciferase expression by the presence of both the A alleles at rs2736108 and rs2736109, but not when either allele is present alone. Our analysis of 345 Australian controls suggests that the A-A haplotype at rs2736108 and rs2736109 occurs with a frequency of 32%, suggesting that this relatively common promoter haplotype may lower the risk of serous epithelial ovarian cancer though decreasing TERT expression.